Project

Part:BBa_K3218006:Design

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-16)


MS2-N55K, a mutant of the bacteriophage MS2 coat protein with enhanced affinity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to design how it will be expressed in our plasmid we used a T2A cleavage site in which eukaryotic ribosomal units are not able to produce a peptide bond between glycine and proline. Furthermore we had to design the gRNAs target with to which side of DNA will be targeted.


Source

MS2 protein is a bacteriophage coat protein

References